Lab 3 Biology 121
Autor: Gabby Gomez • March 20, 2019 • Lab Report • 408 Words (2 Pages) • 569 Views
Name: Gabriella Gomez
Cheek Cell DNA-Lab Report Sheet
Exercise 1 – Isolating Cheek Cell DNA
Analysis of Results
Appearance of Solution | What is happening to the cells/DNA? | |
After lysis buffer | Started off hydrophobic. It clumped together and sank to the bottom of the liquid, after shaking it a few times, it was more cloudy | The individual cells are being split apart by the detergent and salt to isolate the DNA. |
After alcohol added | The alcohol and the solution did not mix creating a small layer. | The solubility of the DNA is being affected by the alcohol. That’s why it stayed at the bottom. |
After alcohol mixed | After mixing, the tube was clear, at the bottom were little floaty string things. | The remaining cells dissolved and all that was left were strands of DNA that you could see with the naked eye. |
Questions:
1. Why is cell lysis the first step in isolating DNA from your cells?
Cell lysis is the first step in isolating DNA because the cells have to be broken down before the DNA can be released and extracted. |
2. Lysis buffer typically contains detergent. Why would this help to lyse the cells?
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