Isolating Cheek Cell Dna
Autor: rikkurai • September 30, 2015 • Lab Report • 283 Words (2 Pages) • 1,912 Views
Name: Erica Smallwood
Cheek Cell DNA-Lab Report Sheet
Exercise 1 – Isolating Cheek Cell DNA
Analysis of Results
Appearance of Solution | What is happening to the cells/DNA? | |
After lysis buffer | The solution had white material floating and is somewhat cloudy | DNA extraction, cells outer layers are breaking open |
After alcohol added | Top layer clear, bottom layer cloudy with white material floating | DNA separated from other molecules in the cell extract and DNA is precipitated. |
After alcohol mixed | Cloudy, saw several stringy white pieces floating around that seemed bouncy in the middle of the tube | DNA precipitated and aggregates until seen by naked eye, other molecules in the cell extract remain dissolved and not visible. |
Questions:
1. Why is cell lysis the first step in isolating DNA from your cells?
Cell lysis is the first step in isolating DNA from your cells because the cells have to be broken down into smaller components to release the DNA. |
2. Lysis buffer typically contains detergent. Why would this help to lyse the cells?
Detergent helps lyse the cells by dissolving and breaking down oils, since most cells are oil-based. |
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